Methods in Nucleic Acids Research

Methods in Nucleic Acids Research

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Methods in Nucleic Acids Research provides extensively referenced overviews of chapter topics, in addition to step-by-step laboratory protocols. Topics include discussions regarding the preparation and assay of antibodies against oligopeptides, RNA footprinting, gel-retardation assays for nucleic acid binding proteins, in vitro transcription and translation assays for studies of eukaryotic gene expression, human genome mapping, forensic analysis of DNA polymorphism, in situ hybridization for the detection of specific RNA, and other methods. Biochemists, molecular biologists, immunologists, cell biologists, and geneticists will find this book invaluable for their research.... steps 1 to 4 as described above; each sample is then desalted with a Sephadex G50 spin column using a modification of the procedures described in the Pharmacia manual. ... A table-top centrifuge (Beckman Model TJ-6) is also suitable.

Title:Methods in Nucleic Acids Research
Author: Gregory W. Warr, James D. Karam, Lee Chao
Publisher:CRC Press - 1990-11-26

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